To check the newly decided sequences in opposition to the topic sequences saved within the databases is a vital job within the bioinformatics. Thankfully, latest survey studies that the state-of-the-art aligners are already quick sufficient to deal with the extremely quantity of quick sequence reads within the cheap time.
Nonetheless, for aligning the lengthy sequence reads (>400 bp) generated by the subsequent era sequencing (NGS) know-how, it’s nonetheless fairly inefficient with current aligners. Moreover, the problem turns into increasingly more severe because the lengths and the quantities of the sequence reads are each protecting growing with the development of the sequencing know-how.
Thus, this can be very pressing for the researchers to reinforce the efficiency of the lengthy learn alignment. On this paper, we suggest a novel FPGA-based system to enhance the effectivity of the lengthy learn mapping. In comparison with the state-of-the-art lengthy learn aligner BWA-SW, our accelerating platform might obtain a excessive efficiency with virtually the identical sensitivity.
Experiments exhibit that, for reads with lengths starting from 512 as much as 4,096 base pairs, the described system obtains a 10x -48x speedup for the bottleneck of the software program. As to the entire mapping process, the FPGA-based platform might obtain a 1.8x -3:3x speedup versus the BWA-SW aligner, decreasing the alignment cycles from weeks to days.
Benchmarking quick sequence mapping instruments.
The event of next-generation sequencing devices has led to the era of tens of millions of quick sequences in a single run. The method of aligning these reads to a reference genome is time consuming and calls for the event of quick and correct alignment instruments.
Nonetheless, the present proposed instruments make totally different compromises between the accuracy and the velocity of mapping. Furthermore, many vital facets are neglected whereas evaluating the efficiency of a newly developed instrument to the cutting-edge.
Due to this fact, there’s a want for an goal analysis methodology that covers all of the facets. On this work, we introduce a benchmarking suite to extensively analyze sequencing instruments with respect to numerous facets and supply an goal comparability.
RESULTS
We utilized our benchmarking checks on 9 well-known mapping instruments, specifically, Bowtie, Bowtie2, BWA, SOAP2, MAQ, RMAP, GSNAP, Novoalign, and mrsFAST (mrFAST) utilizing artificial information and actual RNA-Seq information. MAQ and RMAP are based mostly on constructing hash tables for the reads, whereas the remaining instruments are based mostly on indexing the reference genome.
The benchmarking checks reveal the strengths and weaknesses of every instrument. The outcomes present that no single instrument outperforms all others in all metrics. Nonetheless, Bowtie maintained one of the best throughput for a lot of the checks whereas BWA carried out higher for longer learn lengths. The benchmarking checks will not be restricted to the talked about instruments and could be additional utilized to others.
CONCLUSIONS
The mapping course of continues to be a tough drawback that’s affected by many elements. On this work, we offered a benchmarking suite that reveals and evaluates the various factors affecting the mapping course of. Nonetheless, there is no such thing as a instrument that outperforms all the others in all of the checks. Due to this fact, the top consumer ought to clearly specify his wants so as to select the instrument that gives one of the best outcomes.
Sturdy mapping of polyadenylated and non-polyadenylated RNA 3′ ends at nucleotide decision by 3′-end sequencing.
3′-end poly(A)+ sequencing is an environment friendly and economical methodology for international measurement of mRNA ranges and different poly(A) web site utilization. A standard methodology includes oligo(dT)19V reverse-transcription (RT)-based library preparation and high-throughput sequencing with a customized primer ending in (dT)19.
Whereas nearly all of library merchandise have the primary sequenced nucleotide mirror the bona fide poly(A) web site (pA), a considerable fraction of sequencing reads come up from varied mis-priming occasions. These may end up in incorrect pA web site calls wherever from a number of nucleotides downstream to a number of kilobases upstream from the bona fide pA web site.
Whereas these mis-priming occasions could be mitigated by growing annealing stringency (e.g. growing temperature from 37 °C to 42 °C), they nonetheless persist at an considerable stage (∼10%) and computational strategies should be used to forestall artifactual calls. Right here we current a bioinformatics workflow for exact mapping of poly(A)+ 3′ ends and dealing with of artifacts as a result of oligo(dT) mis-priming and pattern polymorphisms.
We check pA web site calling with three totally different learn mapping applications (STAR, BWA, and BBMap), and present that the way in which wherein every handles terminal mismatches and comfortable clipping has a considerable impression on figuring out right pA websites, with BWA requiring the least post-processing to right artifacts.
We exhibit using this pipeline for mapping pA websites within the mannequin eukaryote S. cerevisiae, and additional apply this know-how to non-polyadenylated transcripts by using in vitro polyadenylation previous to library prep (IVP-seq).
Rabbit polyclonal Pirimiphos antibody |
MBS5307023-025mL |
MyBiosource |
0.25mL |
EUR 595 |
Rabbit polyclonal Pirimiphos antibody |
MBS5307023-5x025mL |
MyBiosource |
5x0.25mL |
EUR 2530 |
Rabbit polyclonal Pirimiphos antibody |
MBS5307024-005mL |
MyBiosource |
0.05mL |
EUR 595 |
Rabbit polyclonal Pirimiphos antibody |
MBS5307024-5x05mL |
MyBiosource |
5x0.5mL |
EUR 2530 |
Chicken anti Pirimiphos |
MBS570383-1mL |
MyBiosource |
1mL |
EUR 720 |
Chicken anti Pirimiphos |
MBS570383-5x1mL |
MyBiosource |
5x1mL |
EUR 3090 |
Pirimiphos |
AS13-2743 |
Agrisera AB |
0,5 mg (0,8 mg/ml) |
EUR 501 |
Pirimiphos antibody |
70-1065 |
Fitzgerald |
500 ul |
EUR 353 |
- Supplied as a purified polyclonal antibody
|
Description: Chicken polyclonal Pirimiphos antibody |
Pirimiphos antibody |
70-1066 |
Fitzgerald |
250 ul |
EUR 330 |
- Supplied as a purified IgG
|
Description: Rabbit polyclonal Pirimiphos antibody |
Pirimiphos antibody |
70-1075 |
Fitzgerald |
50 ul |
EUR 330 |
- Supplied as a purified polyclonal antibody
|
Description: Rabbit polyclonal Pirimiphos antibody |
Pirimiphos-ethyl |
461048 |
MedKoo Biosciences |
250.0mg |
EUR 210 |
- Smiles Code: S=P(OCC)(OC1=NC(N(CC)CC)=NC(C)=C1)OCC
|
Pirimiphos-ethyl |
T25964-10mg |
TargetMol Chemicals |
10mg |
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- SMILES: S=P(OC1=NC(=NC(=C1)C)N(CC)CC)(OCC)OCC
- Formula: C13H24N3O3PS
|
Description: Pirimiphos-ethyl |
Pirimiphos-ethyl |
T25964-1g |
TargetMol Chemicals |
1g |
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- SMILES: S=P(OC1=NC(=NC(=C1)C)N(CC)CC)(OCC)OCC
- Formula: C13H24N3O3PS
|
Description: Pirimiphos-ethyl |
Pirimiphos-ethyl |
T25964-1mg |
TargetMol Chemicals |
1mg |
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- SMILES: S=P(OC1=NC(=NC(=C1)C)N(CC)CC)(OCC)OCC
- Formula: C13H24N3O3PS
|
Description: Pirimiphos-ethyl |
Pirimiphos-ethyl |
T25964-50mg |
TargetMol Chemicals |
50mg |
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- SMILES: S=P(OC1=NC(=NC(=C1)C)N(CC)CC)(OCC)OCC
- Formula: C13H24N3O3PS
|
Description: Pirimiphos-ethyl |
Pirimiphos-ethyl |
T25964-5mg |
TargetMol Chemicals |
5mg |
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- SMILES: S=P(OC1=NC(=NC(=C1)C)N(CC)CC)(OCC)OCC
- Formula: C13H24N3O3PS
|
Description: Pirimiphos-ethyl |
Pirimiphos-ethyl |
MBS5775694-5mg |
MyBiosource |
5(mg |
EUR 915 |
Pirimiphos-ethyl |
MBS5775694-5x5mg |
MyBiosource |
5x5(mg |
EUR 3970 |
Pirimiphos-methyl |
461039 |
MedKoo Biosciences |
250.0mg |
EUR 240 |
- Smiles Code: S=P(OC)(OC1=NC(N(CC)CC)=NC(C)=C1)OC
|
Pirimiphos-methyl |
T20539-10mg |
TargetMol Chemicals |
10mg |
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- SMILES: S=P(OC1=NC(=NC(=C1)C)N(CC)CC)(OC)OC
- Formula: C11H20N3O3PS
|
Description: Pirimiphos-methyl |
Pirimiphos-methyl |
T20539-1g |
TargetMol Chemicals |
1g |
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- SMILES: S=P(OC1=NC(=NC(=C1)C)N(CC)CC)(OC)OC
- Formula: C11H20N3O3PS
|
Description: Pirimiphos-methyl |
Pirimiphos-methyl |
T20539-1mg |
TargetMol Chemicals |
1mg |
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- SMILES: S=P(OC1=NC(=NC(=C1)C)N(CC)CC)(OC)OC
- Formula: C11H20N3O3PS
|
Description: Pirimiphos-methyl |
Pirimiphos-methyl |
T20539-50mg |
TargetMol Chemicals |
50mg |
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- SMILES: S=P(OC1=NC(=NC(=C1)C)N(CC)CC)(OC)OC
- Formula: C11H20N3O3PS
|
Description: Pirimiphos-methyl |
Pirimiphos-methyl |
T20539-5mg |
TargetMol Chemicals |
5mg |
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- SMILES: S=P(OC1=NC(=NC(=C1)C)N(CC)CC)(OC)OC
- Formula: C11H20N3O3PS
|
Description: Pirimiphos-methyl |
Pirimiphos-methyl |
MBS5764773-100mg |
MyBiosource |
100mg |
EUR 155 |
Pirimiphos-methyl |
MBS5764773-50mg |
MyBiosource |
50mg |
EUR 135 |
Pirimiphos-methyl |
MBS5764773-5x100mg |
MyBiosource |
5x100mg |
EUR 540 |
Chicken polyclonal Pirimiphos antibody |
MBS5307022-05mL |
MyBiosource |
0.5mL |
EUR 630 |
Chicken polyclonal Pirimiphos antibody |
MBS5307022-5x05mL |
MyBiosource |
5x0.5mL |
EUR 2675 |
Pirimicarb |
20-abx184758 |
Abbexa |
-
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- Shipped within 1-2 weeks.
|
Pirimicarb |
T19810-10mg |
TargetMol Chemicals |
10mg |
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- SMILES: O=C(OC=1N=C(N=C(C1C)C)N(C)C)N(C)C
- Formula: C11H18N4O2
|
Description: Pirimicarb |
Pirimicarb |
T19810-1g |
TargetMol Chemicals |
1g |
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- SMILES: O=C(OC=1N=C(N=C(C1C)C)N(C)C)N(C)C
- Formula: C11H18N4O2
|
Description: Pirimicarb |
Pirimicarb |
T19810-1mg |
TargetMol Chemicals |
1mg |
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- SMILES: O=C(OC=1N=C(N=C(C1C)C)N(C)C)N(C)C
- Formula: C11H18N4O2
|
Description: Pirimicarb |
Pirimicarb |
T19810-50mg |
TargetMol Chemicals |
50mg |
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- SMILES: O=C(OC=1N=C(N=C(C1C)C)N(C)C)N(C)C
- Formula: C11H18N4O2
|
Description: Pirimicarb |
Pirimicarb |
T19810-5mg |
TargetMol Chemicals |
5mg |
Ask for price |
- SMILES: O=C(OC=1N=C(N=C(C1C)C)N(C)C)N(C)C
- Formula: C11H18N4O2
|
Description: Pirimicarb |
Pirimicarb |
HY-119419 |
MedChemExpress |
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Description: Pirimicarb is a fast-acting selective carbamate insecticide on a wide range of crops including cereals, sugar beet, potatoes, fruits and vegetables. Pirimicarb is an AChE inhibitor and an acaricide[1]. |
Pirimicarb-d6 |
HY-119419S |
MedChemExpress |
1mg |
Ask for price |
Description: Pirimicarb-d6 is the deuterium labeled Diuron. Diuron is a phenylurea herbicide that inhibits photosynthesis by preventing the formation of ATP and NADH. Diuron (2,500 ppm, dietary) increases the incidence of urinary bladder urothelial carcinomas in male |
Rabbit anti-human pirin (iron-binding nuclear protein) polyclonal Antibody |
MBS712777-01mL |
MyBiosource |
0.1mL |
EUR 485 |
Rabbit anti-human pirin (iron-binding nuclear protein) polyclonal Antibody |
MBS712777-5x01mL |
MyBiosource |
5x0.1mL |
EUR 2175 |
Rabbit Pirin ELISA Kit |
MBS037180-INQUIRE |
MyBiosource |
INQUIRE |
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Rabbit Pirin ELISA Kit |
MBS730852-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 5685 |
Rabbit Pirin ELISA Kit |
MBS730852-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 485 |
Rabbit Pirin ELISA Kit |
MBS730852-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 3020 |
Rabbit Pirin ELISA Kit |
MBS730852-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 690 |
Rabbit Pirin (PIR) ELISA Kit |
abx363164-96tests |
Abbexa |
96 tests |
EUR 990 |
- Shipped within 5-12 working days.
|
Rabbit PIR (Pirin) ELISA Kit |
MBS2505857-10x96Tests |
MyBiosource |
10x96Tests |
EUR 4445 |
Rabbit PIR (Pirin) ELISA Kit |
MBS2505857-24Tests |
MyBiosource |
24Tests |
EUR 280 |
Rabbit PIR (Pirin) ELISA Kit |
MBS2505857-48Tests |
MyBiosource |
48Tests |
EUR 495 |
Rabbit PIR (Pirin) ELISA Kit |
MBS2505857-5x96Tests |
MyBiosource |
5x96Tests |
EUR 2480 |
Rabbit PIR (Pirin) ELISA Kit |
MBS2505857-96Tests |
MyBiosource |
96Tests |
EUR 585 |
Rabbit anti-Escherichia coli PIR Polyclonal Antibody |
MBS7166556-INQUIRE |
MyBiosource |
INQUIRE |
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Rabbit anti-Oryza sativa subsp. indica (Rice) PIR7B Polyclonal Antibody |
MBS7167596-INQUIRE |
MyBiosource |
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Rabbit anti-Oryza sativa subsp. indica (Rice) PIR7A Polyclonal Antibody |
MBS7167703-INQUIRE |
MyBiosource |
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Rabbit anti-Oryza sativa subsp. japonica (Rice) PIR7A Polyclonal Antibody |
MBS7188748-INQUIRE |
MyBiosource |
INQUIRE |
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Rabbit anti-Oryza sativa subsp. japonica (Rice) PIR7B Polyclonal Antibody |
MBS7189247-INQUIRE |
MyBiosource |
INQUIRE |
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Rabbit anti-Saccharomyces cerevisiae (strain AWRI1631)(Baker's yeast) PIR5 Polyclonal Antibody |
MBS7172199-INQUIRE |
MyBiosource |
INQUIRE |
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Rabbit anti-Saccharomyces cerevisiae (strain YJM789)(Baker's yeast) PIR1 Polyclonal Antibody |
MBS7168162-INQUIRE |
MyBiosource |
INQUIRE |
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Rabbit anti-Saccharomyces cerevisiae (strain YJM789)(Baker's yeast) PIR3 Polyclonal Antibody |
MBS7168314-INQUIRE |
MyBiosource |
INQUIRE |
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Rabbit anti-Saccharomyces cerevisiae (strain YJM789)(Baker's yeast) PIR5 Polyclonal Antibody |
MBS7168355-INQUIRE |
MyBiosource |
INQUIRE |
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Rabbit anti-Saccharomyces cerevisiae (strain RM11-1a)(Baker's yeast) PIR3 Polyclonal Antibody |
MBS7170621-INQUIRE |
MyBiosource |
INQUIRE |
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Rabbit anti-Saccharomyces cerevisiae (strain RM11-1a)(Baker's yeast) PIR5 Polyclonal Antibody |
MBS7170834-INQUIRE |
MyBiosource |
INQUIRE |
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Rabbit anti-Saccharomyces cerevisiae (strain RM11-1a)(Baker's yeast) PIR1 Polyclonal Antibody |
MBS7170836-INQUIRE |
MyBiosource |
INQUIRE |
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Rabbit Anti BSA |
E61I026 |
EnoGene |
0.1mg |
EUR 225 |
Description: Available in various conjugation types. |
Rabbit Anti BSA |
MBS8576923-01mg |
MyBiosource |
0.1mg |
EUR 305 |
Rabbit Anti BSA |
MBS8576923-01mLAF405L |
MyBiosource |
0.1mL(AF405L) |
EUR 465 |
Rabbit Anti BSA |
MBS8576923-01mLAF405S |
MyBiosource |
0.1mL(AF405S) |
EUR 465 |
Rabbit Anti BSA |
MBS8576923-01mLAF610 |
MyBiosource |
0.1mL(AF610) |
EUR 465 |
Rabbit Anti BSA |
MBS8576923-01mLAF635 |
MyBiosource |
0.1mL(AF635) |
EUR 465 |
RABBIT ANTI HRK |
MBS221467-01mg |
MyBiosource |
0.1mg |
EUR 545 |
RABBIT ANTI HRK |
MBS221467-5x01mg |
MyBiosource |
5x0.1mg |
EUR 2275 |
RABBIT ANTI BIM |
MBS221821-01mg |
MyBiosource |
0.1mg |
EUR 440 |
RABBIT ANTI BIM |
MBS221821-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1805 |
RABBIT ANTI HRK |
MBS223071-005mg |
MyBiosource |
0.05mg |
EUR 355 |
RABBIT ANTI HRK |
MBS223071-5x005mg |
MyBiosource |
5x0.05mg |
EUR 1425 |
RABBIT ANTI DAI |
MBS223498-01mg |
MyBiosource |
0.1mg |
EUR 460 |
RABBIT ANTI DAI |
MBS223498-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1905 |
RABBIT ANTI DAI |
MBS223548-005mg |
MyBiosource |
0.05mg |
EUR 290 |
RABBIT ANTI DAI |
MBS223548-5x005mg |
MyBiosource |
5x0.05mg |
EUR 1125 |
RABBIT ANTI SRC |
MBS226038-005mL |
MyBiosource |
0.05mL |
EUR 450 |
RABBIT ANTI SRC |
MBS226038-5x005mL |
MyBiosource |
5x0.05mL |
EUR 1840 |
RABBIT ANTI MIF |
MBS224848-005mg |
MyBiosource |
0.05mg |
EUR 440 |
RABBIT ANTI MIF |
MBS224848-5x005mg |
MyBiosource |
5x0.05mg |
EUR 1805 |
RABBIT ANTI BAK |
MBS224877-005mg |
MyBiosource |
0.05mg |
EUR 440 |
RABBIT ANTI BAK |
MBS224877-5x005mg |
MyBiosource |
5x0.05mg |
EUR 1805 |
Rabbit anti Sp1 |
MBS225058-01mg |
MyBiosource |
0.1mg |
EUR 455 |
Rabbit anti Sp1 |
MBS225058-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1870 |
Rabbit anti BAX |
MBS225064-01mg |
MyBiosource |
0.1mg |
EUR 440 |
Rabbit anti BAX |
MBS225064-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1805 |
Rabbit anti BAD |
MBS225065-005mL |
MyBiosource |
0.05mL |
EUR 455 |
Rabbit anti BAD |
MBS225065-5x005mL |
MyBiosource |
5x0.05mL |
EUR 1870 |
Rabbit anti ATM |
MBS225084-005mL |
MyBiosource |
0.05mL |
EUR 455 |
Rabbit anti ATM |
MBS225084-5x005mL |
MyBiosource |
5x0.05mL |
EUR 1870 |
Rabbit anti FGR |
MBS225109-005mL |
MyBiosource |
0.05mL |
EUR 455 |
Rabbit anti FGR |
MBS225109-5x005mL |
MyBiosource |
5x0.05mL |
EUR 1870 |
Rabbit anti Gbl |
MBS225110-005mL |
MyBiosource |
0.05mL |
EUR 455 |
Rabbit anti Gbl |
MBS225110-5x005mL |
MyBiosource |
5x0.05mL |
EUR 1870 |
Rabbit anti Hck |
MBS225115-005mL |
MyBiosource |
0.05mL |
EUR 455 |
Rabbit anti Hck |
MBS225115-5x005mL |
MyBiosource |
5x0.05mL |
EUR 1870 |
Rabbit anti Lck |
MBS225134-005mL |
MyBiosource |
0.05mL |
EUR 455 |
Rabbit anti Lck |
MBS225134-5x005mL |
MyBiosource |
5x0.05mL |
EUR 1870 |
Rabbit anti Lyn |
MBS225136-005mL |
MyBiosource |
0.05mL |
EUR 455 |
Rabbit anti Lyn |
MBS225136-5x005mL |
MyBiosource |
5x0.05mL |
EUR 1870 |
Rabbit anti NAK |
MBS225140-005mL |
MyBiosource |
0.05mL |
EUR 455 |
Rabbit anti NAK |
MBS225140-5x005mL |
MyBiosource |
5x0.05mL |
EUR 1870 |
Rabbit anti SYK |
MBS225168-005mL |
MyBiosource |
0.05mL |
EUR 455 |
Rabbit anti SYK |
MBS225168-5x005mL |
MyBiosource |
5x0.05mL |
EUR 1870 |
Rabbit anti VHL |
MBS225176-005mL |
MyBiosource |
0.05mL |
EUR 455 |
Rabbit anti VHL |
MBS225176-5x005mL |
MyBiosource |
5x0.05mL |
EUR 1870 |
Rabbit anti BAX |
MBS225327-005mL |
MyBiosource |
0.05mL |
EUR 455 |
Rabbit anti BAX |
MBS225327-5x005mL |
MyBiosource |
5x0.05mL |
EUR 1870 |
Rabbit anti BNP |
MBS315608-01mL |
MyBiosource |
0.1mL |
EUR 640 |
Rabbit anti BNP |
MBS315608-5x01mL |
MyBiosource |
5x0.1mL |
EUR 2695 |
Rabbit anti BNP |
MBS315609-01mL |
MyBiosource |
0.1mL |
EUR 1590 |
Rabbit anti BNP |
MBS315609-5x01mL |
MyBiosource |
5x0.1mL |
EUR 6985 |
Rabbit anti PSA |
MBS315621-5x1mL |
MyBiosource |
5x1mL |
EUR 1280 |
Rabbit anti tPA |
MBS315815-02mg |
MyBiosource |
0.2mg |
EUR 610 |
Rabbit anti tPA |
MBS315815-5x02mg |
MyBiosource |
5x0.2mg |
EUR 2570 |
RABBIT ANTI FITC |
MBS220849-5x1mL |
MyBiosource |
5x1mL |
EUR 1555 |
RABBIT ANTI FITC |
MBS220850-5x1mg |
MyBiosource |
5x1mg |
EUR 2890 |
RABBIT ANTI TSC1 |
MBS221310-01mg |
MyBiosource |
0.1mg |
EUR 415 |
RABBIT ANTI TSC1 |
MBS221310-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1690 |
RABBIT ANTI NOXA |
MBS221324-01mg |
MyBiosource |
0.1mg |
EUR 455 |
RABBIT ANTI NOXA |
MBS221324-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1870 |
RABBIT ANTI SLUG |
MBS221474-01mg |
MyBiosource |
0.1mg |
EUR 470 |
RABBIT ANTI SLUG |
MBS221474-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1935 |
RABBIT ANTI MADD |
MBS221589-01mg |
MyBiosource |
0.1mg |
EUR 565 |
RABBIT ANTI MADD |
MBS221589-5x01mg |
MyBiosource |
5x0.1mg |
EUR 2360 |
RABBIT ANTI DAD1 |
MBS221828-01mg |
MyBiosource |
0.1mg |
EUR 565 |
RABBIT ANTI DAD1 |
MBS221828-5x01mg |
MyBiosource |
5x0.1mg |
EUR 2360 |
RABBIT ANTI PCNA |
MBS222883-005mg |
MyBiosource |
0.05mg |
EUR 750 |
RABBIT ANTI PCNA |
MBS222883-5x005mg |
MyBiosource |
5x0.05mg |
EUR 3210 |
RABBIT ANTI GLI3 |
MBS222908-005mg |
MyBiosource |
0.05mg |
EUR 660 |
RABBIT ANTI GLI3 |
MBS222908-5x005mg |
MyBiosource |
5x0.05mg |
EUR 2795 |
RABBIT ANTI SLPI |
MBS223103-01mg |
MyBiosource |
0.1mg |
EUR 465 |
RABBIT ANTI SLPI |
MBS223103-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1915 |
RABBIT ANTI RIP3 |
MBS223753-01mg |
MyBiosource |
0.1mg |
EUR 470 |
RABBIT ANTI RIP3 |
MBS223753-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1935 |
RABBIT ANTI VISA |
MBS223807-01mg |
MyBiosource |
0.1mg |
EUR 440 |
RABBIT ANTI VISA |
MBS223807-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1805 |
RABBIT ANTI TEM7 |
MBS223982-01mg |
MyBiosource |
0.1mg |
EUR 455 |
RABBIT ANTI TEM7 |
MBS223982-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1870 |
RABBIT ANTI EGR1 |
MBS224123-005mg |
MyBiosource |
0.05mg |
EUR 750 |
RABBIT ANTI EGR1 |
MBS224123-5x005mg |
MyBiosource |
5x0.05mg |
EUR 3210 |
RABBIT ANTI ATG3 |
MBS224303-01mg |
MyBiosource |
0.1mg |
EUR 565 |
RABBIT ANTI ATG3 |
MBS224303-5x01mg |
MyBiosource |
5x0.1mg |
EUR 2360 |
RABBIT ANTI eNOS |
MBS224913-005mg |
MyBiosource |
0.05mg |
EUR 455 |
RABBIT ANTI eNOS |
MBS224913-5x005mg |
MyBiosource |
5x0.05mg |
EUR 1870 |
As proof of precept, we present {that a} fraction of tRNAs harbor CCU 3′ tails as an alternative of the canonical CCA tail, and globally determine 3′ ends of splicing intermediates arising from inefficiently spliced transcripts.