Genome-wide RAD sequencing data provide unprecedented resolution of the phylogeny of temperate bamboos.

Genome-wide RAD sequencing data provide unprecedented resolution of the phylogeny of temperate bamboos.

The temperate bamboos (tribe Arundinarieae, Poaceae) are strongly supported as monophyly in latest molecular research, however taxonomic delineation and phylogenetic relationships inside the tribe lack decision. Right here, we sampled 39 species (36 temperate bamboos and three outgroups) for restriction-site related DNA sequencing (RAD-seq) with an emphasis on Phyllostachys clade and associated clades.

Utilizing the biggest information matrix for the bamboos up to now, we have been in a position to infer phylogenetic relationships with unparalleled decision. The Phyllostachys, Shibataea, and Arundinaria clades outlined from plastid phylogeny, weren’t supported as monophyletic group. Nonetheless, the RAD-seq phylogeny largely agreed with the morphology-based taxonomy, with two clades having leptomorph rhizomes strongly supported as monophyletic group.

We additionally explored two approaches, BWA-GATK (a mapping system) and Stacks (a grouping system), for variations in SNP calling and phylogeny inference. For a similar stage of lacking information, the BWA-GATK pipeline produced way more SNPs as compared with Stacks.

Phylogenetic analyses of the biggest information matrices from each pipelines, utilizing concatenation and coalescent strategies supplied related tree topologies, regardless of the presence of lacking information. Our research demonstrates the utility of RAD-seq information for elucidating phylogenetic relationships between genera and better taxonomic ranges on this essential however phylogenetically difficult group.

Single Nucleotide Polymorphism Identification in Polyploids: A Evaluate, Instance, and Suggestions.

Understanding the connection between genotype and phenotype is a significant organic query and having the ability to predict phenotypes based mostly on molecular genotypes is integral to molecular breeding. Entire-genome duplications have formed the historical past of all flowering vegetation and current challenges to elucidating the connection between genotype and phenotype, particularly in neopolyploid species.

Though single nucleotide polymorphisms (SNPs) have change into fashionable instruments for genetic mapping, discovery and utility of SNPs in polyploids has been tough. Right here, we summarize frequent experimental approaches to SNP calling, highlighting latest polyploid successes.

To look at the influence of software program alternative on these analyses, we referred to as SNPs amongst 5 peanut genotypes utilizing totally different alignment packages (BWA-mem and Bowtie 2) and variant callers (SAMtools, GATK, and Freebayes). Alignments produced by Bowtie 2 and BWA-mem and analyzed in SAMtools shared 24.5% concordant SNPs, and SAMtools, GATK, and Freebayes shared 1.4% concordant SNPs.

A subsequent evaluation of simulated Brassica napus chromosome 1A and 1C genotypes demonstrated that, of the three software program packages, SAMtools carried out with the best sensitivity and specificity on Bowtie 2 alignments. These outcomes, nonetheless, are prone to differ amongst species, and we subsequently suggest a collection of finest practices for SNP calling in polyploids.

Stampy: a statistical algorithm for delicate and quick mapping of Illumina sequence reads.

Excessive-volume sequencing of DNA and RNA is now inside attain of any analysis laboratory and is shortly turning into established as a key analysis device. In lots of workflows, every of the brief sequences (“reads”) ensuing from a sequencing run are first “mapped” (aligned) to a reference sequence to deduce the learn from which the genomic location derived, a difficult job due to the excessive information volumes and infrequently massive genomes.

Present learn mapping software program excel in both pace (e.g., BWA, Bowtie, ELAND) or sensitivity (e.g., Novoalign), however not in each. As well as, efficiency usually deteriorates within the presence of sequence variation, significantly so for brief insertions and deletions (indels).

Right here, we current a learn mapper, Stampy, which makes use of a hybrid mapping algorithm and an in depth statistical mannequin to realize each pace and sensitivity, significantly when reads embody sequence variation. This leads to a better useable sequence yield and improved accuracy in comparison with that of present software program.

 Genome-wide RAD sequencing data provide unprecedented resolution of the phylogeny of temperate bamboos.

Suitability of Totally different Mapping Algorithms for Genome-wide Polymorphism Scans with Pool-Seq Knowledge.

The price-effectiveness of sequencing swimming pools of people (Pool-Seq) supplies the premise for the recognition and widespread use of this technique for a lot of analysis questions, starting from unraveling the genetic foundation of complicated traits, to the clonal evolution of most cancers cells.

As a result of the accuracy of Pool-Seq could possibly be affected by many potential sources of error, a number of research have decided, for instance, the affect of sequencing expertise, the library preparation protocol, and mapping parameters. Nonetheless, the influence of the mapping instruments has not but been evaluated. Utilizing simulated and actual Pool-Seq information, we reveal a considerable influence of the mapping instruments, resulting in attribute false positives in genome-wide scans.

The issue of false positives was significantly pronounced when information with totally different learn lengths and insert sizes have been in contrast. Out of 14 evaluated algorithms novoalign, bwa mem and clc4 are most fitted for mapping Pool-Seq information. Nonetheless, no single algorithm is enough for avoiding all false positives.

We present that the intersection of the outcomes of two mapping algorithms supplies a easy, but efficient, technique to eradicate false positives.

Chimpanzee Anti-Adenovirus Hexon Protein (hAd5 hAd5 hxn) IgG negative control serum

950-150-02N 1 ml
EUR 164

Recombinant Chimpanzee Alpha-fetoprotein Protein

VAng-2994Lsx-1mg 1 mg
EUR 3748
Description: Recombinant Chimpanzee Alpha-fetoprotein Antigen is expressed in E. coli. (Uniprot ID: Q28789)

Recombinant Chimpanzee Alpha-fetoprotein Protein

VAng-2994Lsx-200g 200 µg
EUR 1343
Description: Recombinant Chimpanzee Alpha-fetoprotein Antigen is expressed in E. coli. (Uniprot ID: Q28789)

Recombinant Chimpanzee Alpha-fetoprotein Protein

VAng-2994Lsx-500g 500 µg
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Chimpanzee IgG (non-immune)-Biotin conjugate

20012-Ch-B 100 ug
EUR 286

Chimpanzee IgG (non-immune, isotype control), purified

20012-Ch-1 1 mg
EUR 225

Recombinant Chimpanzee Glypican-3 Protein, His, Mammal-100ug

QP10081-ma-100ug 100ug
EUR 1178

Recombinant Chimpanzee Glypican-3 Protein, His, Mammal-20ug

QP10081-ma-20ug 20ug
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Recombinant Chimpanzee Glypican-3 Protein, His, Mammal-50ug

QP10081-ma-50ug 50ug
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Recombinant Chimpanzee Activin A Protein (aa 34-463)

VAng-2810Lsx-1mg 1 mg
EUR 5247
Description: Recombinant Chimpanzee Activin A was expressed in E. coli. (Uniprot ID: Q0MQG5)

Recombinant Chimpanzee Activin A Protein (aa 34-463)

VAng-2810Lsx-200g 200 µg
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Description: Recombinant Chimpanzee Activin A was expressed in E. coli. (Uniprot ID: Q0MQG5)

Recombinant Chimpanzee Activin A Protein (aa 34-463)

VAng-2810Lsx-50g 50 µg
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Recombinant Chimpanzee LTB/ TNFSF3/ Lymphotoxin beta Protein, His, E.coli-100ug

QP7788-ec-100ug 100ug
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Recombinant Chimpanzee LTB/ TNFSF3/ Lymphotoxin beta Protein, His, E.coli-1mg

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Recombinant Chimpanzee LTB/ TNFSF3/ Lymphotoxin beta Protein, His, E.coli-200ug

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Recombinant Chimpanzee LTB/ TNFSF3/ Lymphotoxin beta Protein, His, E.coli-500ug

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Recombinant Chimpanzee LTB/ TNFSF3/ Lymphotoxin beta Protein, His, E.coli-50ug

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Equine Serum

EQS001 500 ml
EUR 200

Cow Serum

abx098251-100ml 100 ml
EUR 342
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Fish Serum

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Goat Serum

abx098258-500ml 500 ml
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abx098259-500ml 500 ml
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Horse Serum

abx098260-100ml 100 ml
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Mouse Serum

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Mouse Serum

20-abx098261
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abx098261-5L 5 L
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abx098262-1ml 1 ml
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abx098267-10ml 10 ml
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abx098268-100ml 100 ml
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Canine Serum

88R-D005S 50 ml
EUR 154
Description: Non Sterile Canine Serum

Rabbit Serum

1267-1
EUR 157

Chicken Serum

SER001 500 ml
EUR 265

Mouse Serum Amyloid P Component (SAP) Reference serum

SAP12-RS 100 ul
EUR 324

Rabbit Serum (Sterile)

20-abx800002
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abx800002-1L 1 L
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Sheep Serum (Sterile)

20-abx800019
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Sheep Serum (Sterile)

abx800019-1L 1 L
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Goat Serum (Sterile)

20-abx800034
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Goat Serum (Sterile)

abx800034-1L 1 L
EUR 926
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Horse Serum (Sterile)

20-abx800049
  • EUR 829.00
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Horse Serum (Sterile)

abx800049-1L 1 L
EUR 1316
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Donkey Serum (Sterile)

20-abx800064
  • EUR 1358.00
  • EUR 1803.00
  • EUR 746.00
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Donkey Serum (Sterile)

abx800064-1L 1 L
EUR 2221
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Camel Serum (Sterile)

20-abx800079
  • EUR 1511.00
  • EUR 342.00
  • EUR 3460.00
  • EUR 913.00
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Camel Serum (Sterile)

abx800079-1L 1 L
EUR 5924
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Dog Serum (Sterile)

20-abx800109
  • EUR 1288.00
  • EUR 2973.00
  • EUR 759.00
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Dog Serum (Sterile)

abx800109-1L 1 L
EUR 4935
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Chicken Serum (Sterile)

20-abx800124
  • EUR 746.00
  • EUR 1609.00
  • EUR 467.00
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Chicken Serum (Sterile)

abx800124-1L 1 L
EUR 2583
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Bovine Serum Albumin

HY-D0842 10g
EUR 187

Bovine Serum Albumin

GE1877-100G 100 g
EUR 158

Bovine Serum Albumin

GE1877-10G 10 g
EUR 54

Bovine Serum Albumin

GE1877-250G 250 g
EUR 285

Bovine Serum Albumin

GE1877-25G 25 g
EUR 70

Bovine Serum Albumin

GE1877-500G 500 g
EUR 500

Bovine Serum Albumin

GE1877-50G 50 g
EUR 102

Newborn Calf Serum

F0603-050 500ml
EUR 151

Doner Horse Serum

F0605-050 500ml
EUR 151

Albumin Human Serum

abx082261-250mg 250 mg
EUR 203
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Normal Bovine Serum

abx098263-100ml 100 ml
EUR 258
  • Shipped within 5-10 working days.

Serum Albumin Protein

20-abx263082
  • EUR 230.00
  • EUR 328.00
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Serum Albumin Protein

abx263082-5gr 5 gr
EUR 1066
  • Shipped within 5-10 working days.

Defibrinated Human Serum

90-1010 10 ml
EUR 127
Description: Defibrinated Human Serum for use in Controls

Normal Goat Serum

9067 50 ml
EUR 100.75
Description: Normal Goat Serum

Normal Human Serum

90R-1002 10 ml
EUR 254
Description: Normal Human Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Steroid Free Serum

90R-1007 50 mL
EUR 392
Description: Steroid Free Human Serum

Normal Goat Serum

88-B9999G000-S0 10 ml
EUR 133
Description: Control Normal Goat serum

Normal Canine Serum

88-NC25 100 ml
EUR 606
Description: Normal Canine Serum

Normal Horse Serum

88-NE21 100 ml
EUR 30
Description: Control Normal Horse Serum

Normal Goat Serum

88-NG22 500 ml
EUR 30
Description: Control Normal Goat Serum

Normal Goat Serum

88-NG22S 500 ml
EUR 191
Description: Control Normal Goat Serum

Normal Mouse Serum

88-NM35 50 ml
EUR 516
Description: Normal Mouse Serum

Normal Pig Serum

88-NP 500 ml
EUR 243
Description: Normal Pig Serum collected from pigs in the USA

Normal Rabbit Serum

88-NR50 100 ml
EUR 150
Description: Normal Rabbit Serum

Normal Rat Serum

88-NR51 50 ml
EUR 599
Description: Normal Rat Serum

Normal Sheep Serum

88-NS55 100 ml
EUR 126
Description: Normal Sheep Serum

Normal Mouse Serum

88R-1002 5 ml
EUR 203
Description: Normal Mouse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Rabbit Serum

88R-1004 10 ml
EUR 206
Description: Normal Rabbit Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Goat Serum

88R-1006 10 ml
EUR 182
Description: Normal Goat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Sheep Serum

88R-1008 10 ml
EUR 182
Description: Normal Sheep Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Rat Serum

88R-1010 5 ml
EUR 202
Description: Normal Rat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Chicken Serum

88R-1014 5 ml
EUR 176
Description: Normal Chicken Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Horse Serum

88R-1020 10 ml
EUR 182
Description: Normal Horse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Donkey Serum

88R-1022 10 ml
EUR 182
Description: Normal Donkey Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Pig Serum

88R-1023 2 ml
EUR 116
Description: Normal Pig (Swine) Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Cat Serum

88R-1026 10 ml
EUR 250
Description: Normal Cat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Dog Serum

88R-1028 10 ml
EUR 251
Description: Normal Dog Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Equine Serum Albumin

88R-1048 100 gram
EUR 2509
Description: Equine Serum Albumin standard grade powder.

Normal Bovine Serum

88R-B001 100 ml
EUR 165
Description: Control Normal Bovine Serum

Normal Donkey Serum

88R-D001 100 ml
EUR 179
Description: Control Normal Donkey Serum

Normal Donkey Serum

88R-D003 10 ml
EUR 149
Description: Control Normal Donkey Serum

Normal Goat Serum

88R-NG001 50 ml
EUR 117
Description: Normal Goat Serum for use as a control or blocking reagent

Normal Pig Serum

88R-P004 1 liter
EUR 673
Description: Control Normal Pig Serum

Normal Sheep Serum

88R-S002 100 ml
EUR 165
Description: Control Normal Sheep Serum

Normal Rabbit Serum

89R-RM001 20 mg
EUR 392
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum

89R-RM002 5 ml
EUR 241
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum

89R-RR001 20 mg
EUR 349
Description: Normal Rabbit Serum (Rat cell adsorbed)

Normal Rabbit Serum

89R-RR002 5 ml
EUR 241
Description: Normal Rabbit Serum (Rat cell adsorbed)

Hemopexin, Rat Serum

7545-100
EUR 283

Serum Thymic Factor

5-01924 4 x 5mg Ask for price

Human Serum Albumin

4016-10MG
EUR 495

Human Serum Albumin

4016-1MG
EUR 180

Serum Albumin Antibody

48145-100ul 100ul
EUR 333

Serum Albumin Antibody

48145-50ul 50ul
EUR 239

Human Serum Albumin

30R-2429 10 mg
EUR 597
Description: Purified native Human Serum Albumin

Rat Serum Albumin

30R-3297 10 mg
EUR 187
Description: Purified native Rat Serum Albumin

Pigeon Serum Albumin

30R-3298 10 mg
EUR 327
Description: Purified native Pigeon Serum Albumin

Chicken Serum Albumin

30R-3299 10 mg
EUR 187
Description: Purified native Chicken Serum Albumin

Horse Serum Albumin

30R-3300 10 mg
EUR 257
Description: Purified native Horse Serum Albumin

Sheep Serum Albumin

30R-3301 10 mg
EUR 257
Description: Purified native Sheep Serum Albumin

Human Serum Albumin

30R-3302 10 mg
EUR 349
Description: Purified native Human Serum Albumin

Rabbit Serum Albumin

30R-3303 10 mg
EUR 187
Description: Purified native Rabbit Serum Albumin

Mouse Serum Albumin

30R-3304 10 mg
EUR 187
Description: Purified native Mouse Serum Albumin

Goat Serum Albumin

30R-3305 10 mg
EUR 257
Description: Purified native Goat Serum Albumin

Porcine Serum Albumin

30R-3307 10 mg
EUR 187
Description: Purified native Porcine serum albumin

Canine Serum Albumin

30R-3308 10 mg
EUR 327
Description: Purified native Canine Serum Albumin

Hamster Complement Serum

32R-CH001 5 ml
EUR 424
Description: Purified normal Hamster Complement isolated from blood

Mouse Complement Serum

32R-CM001 5 ml
EUR 435
Description: Frozen high activity mouse complement serum

Rabbit Complement Serum

32R-CR001 25 ml
EUR 241
Description: Purifed Rabbit Complement isolated from blood of New Zealand white rabbits

Rat Complement Serum

32R-CR005 5 ml
EUR 435
Description: Purifed Rat Complement isolated from blood of normal rats

Goat Serum antibody

20-S1121G000-V0 10 ml
EUR 133
Description: Guinea pig polyclonal Goat Serum antibody

Goat Serum antibody

20-S1121GN00-D0 10 ml
EUR 133
Description: Guinea pig polyclonal Goat Serum antibody

Bovine Serum Albumin

7-04585 10gr Ask for price

Bovine Serum Albumin

7-04586 50gr Ask for price

Bovine Serum Albumin

7-04587 100gr Ask for price

Human Serum Albumin

7-05389 100mg Ask for price

Human Serum Albumin

7-05390 500mg Ask for price

Human Serum Albumin

7-05391 5gram Ask for price

ORANGE SERUM AGAR

O15-101-10kg 10 kg
EUR 1729

ORANGE SERUM AGAR

O15-101-2Kg 2 Kg
EUR 415

ORANGE SERUM AGAR

O15-101-500g 500 g
EUR 149

ORANGE SERUM BROTH

O15-106-10kg 10 kg
EUR 1033

ORANGE SERUM BROTH

O15-106-2Kg 2 Kg
EUR 264

ORANGE SERUM BROTH

O15-106-500g 500 g
EUR 108

Human Serum AB

SC00B14 100 ml
EUR 362

Serum Albumin ELISA Kit| Bovine Serum Albumin ELISA Kit

EF012036 96 Tests
EUR 689

Non-sterile canine serum

CAS05-0100 100 ml
EUR 335.4
  • Non-sterile canine serum is indicated as RUO. Do not use on humans.

Non-sterile canine serum

CAS05-0500 500 ml
EUR 508.3
  • Non-sterile canine serum is indicated as RUO. Do not use on humans.

Human serum C4-depleted

C415-DS-1 1 ml
EUR 408

Serum Albumin Conjugated Antibody

C48145 100ul
EUR 397

Rabbit Serum (Non-Sterile

20-abx800001
  • EUR 1080.00
  • EUR 2472.00
  • EUR 662.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Rabbit Serum (Non-Sterile

abx800001-1L 1 L
EUR 4281
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Sheep Serum (Non-Sterile)

20-abx800018
  • EUR 481.00
  • EUR 592.00
  • EUR 370.00
  • 100 ml
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  • 50 ml
  • Shipped within 1-5 working days.

Sheep Serum (Non-Sterile)

abx800018-1L 1 L
EUR 746
  • Shipped within 1-5 working days.

Goat Serum (Non-Sterile)

20-abx800033
  • EUR 495.00
  • EUR 606.00
  • EUR 370.00
  • 100 ml
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  • 50 ml
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Goat Serum (Non-Sterile)

abx800033-1L 1 L
EUR 759
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Mouse Serum Albumin Protein

20-abx600021
  • EUR 926.00
  • EUR 314.00
  • EUR 3975.00
  • EUR 244.00
  • 100 mg
  • 10 mg
  • 1 g
  • 1 mg
  • Shipped within 1 month.

Horse Serum (Non-Sterile)

20-abx800048
  • EUR 648.00
  • EUR 829.00
  • EUR 467.00
  • 100 ml
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  • 50 ml
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Horse Serum (Non-Sterile)

abx800048-1L 1 L
EUR 1080
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Donkey Serum (Non-Sterile)

20-abx800063
  • EUR 1038.00
  • EUR 1372.00
  • EUR 648.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Donkey Serum (Non-Sterile)

abx800063-1L 1 L
EUR 1803
  • Shipped within 1-5 working days.

Camel Serum (Non-Sterile)

20-abx800078
  • EUR 1149.00
  • EUR 2722.00
  • EUR 787.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Camel Serum (Non-Sterile)

abx800078-1L 1 L
EUR 4699
  • Shipped within 1-5 working days.

Beagle dog Serum Sterile

20-abx800094
  • EUR 1901.00
  • EUR 4532.00
  • EUR 1080.00
  • 100 ml
  • 500 ml
  • 50 ml
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Beagle dog Serum Sterile

abx800094-1L 1 L
EUR 7580
  • Shipped within 1-5 working days.

Dog Serum (Non-Sterile)

20-abx800108
  • EUR 996.00
  • EUR 2263.00
  • EUR 620.00
  • 100 ml
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  • 50 ml
  • Shipped within 1-5 working days.

Dog Serum (Non-Sterile)

abx800108-1L 1 L
EUR 3919
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Chicken Serum (Non-Sterile)

20-abx800123
  • EUR 578.00
  • EUR 1233.00
  • EUR 398.00
  • 100 ml
  • 500 ml
  • 50 ml
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Chicken Serum (Non-Sterile)

abx800123-1L 1 L
EUR 2054
  • Shipped within 1-5 working days.

Bovine Serum Albumin Standard

AR0191 20mL
EUR 70

Normal goat serum(blocking)

AR1009 100mL
EUR 131

Non-sterile calf serum

BCSU05-0100 100 ml
EUR 145.6
  • Non-sterile calf serum is indicated as RUO. Do not use on humans.

We suggest that the implementation of a constant Pool-Seq bioinformatics pipeline, constructing on the suggestions of this research, can considerably enhance the reliability of Pool-Seq outcomes, specifically when libraries generated with totally different protocols are being in contrast.

 

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